› 5hao

X-ray diffraction
2.18Å resolution

Structure function studies of R. palustris RubisCO (M331A mutant; CABP-bound)

Released:
DOI: 10.2210/pdb5hao/pdb
PDB entry 5hao coloured by chain, front view.
PDB entry 5hao coloured by chain, side view.
PDB entry 5hao coloured by chain, top view.
Source organism: Rhodopseudomonas palustris
Entry authors: Arbing MA, Shin A, Satagopan S, North JA, Tabita FR

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose bisphosphate carboxylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 481 amino acids
Theoretical weight: 52.7 KDa
Source organism: Rhodopseudomonas palustris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6N0W9 (Residues: 1-461; Coverage: 100%)
Gene names: RPA4641, cbbM
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CAP 6 x CAP
Ligand MG 6 x MG
1 modified residue:
Ligand KCX 6 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 75.75Å b: 100.12Å c: 100.31Å
α: 112.82° β: 108.14° γ: 88.76°
R-values:
R R work R free
0.205 0.201 0.233
Expression system: Escherichia coli BL21(DE3)

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