› 5gug

X-ray diffraction
7.4Å resolution

Crystal structure of inositol 1,4,5-trisphosphate receptor large cytosolic domain with inositol 1,4,5-trisphosphate

Released:
DOI: 10.2210/pdb5gug/pdb
PDB entry 5gug coloured by chain, front view.
PDB entry 5gug coloured by chain, side view.
PDB entry 5gug coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
IP3-mediated gating mechanism of the IP3 receptor revealed by mutagenesis and X-ray crystallography.
Hamada K, Miyatake H, Terauchi A, Mikoshiba K
Proc Natl Acad Sci U S A 114 4661-4666 (2017)
PMID: 28416699

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145968 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 2217 amino acids
Theoretical weight: 252.38 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11881 (Residues: 1-2217; Coverage: 81%)
Gene names: Insp3r, Itpr1, Pcd6, Pcp1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand I3P 2 x I3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C2221
Unit cell:
a: 211.988Å b: 223.488Å c: 319.869Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.359 0.356 0.416
Expression system: Spodoptera frugiperda

Quick links

Citations

16 review citations

Intracellular calcium release channels: an update.
Santulli et al. (2017)
15 more

3 mentions without citation

IP3-mediated gating mechanism of the IP3 receptor revealed by mutagenesis and X-ray crystallography.
Hamada et al. (2017)
2 more