› 5ghw

X-ray diffraction
2.4Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235032 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB 10E8 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 239 amino acids
Theoretical weight: 25.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB 10E8 LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 36 amino acids
Theoretical weight: 4.62 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 666-690; Coverage: 3%)
Gene name: env

Ligands and Environments

1 bound ligand:
Ligand PO4 7 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: I222
Unit cell:
a: 66.633Å b: 81.231Å c: 254.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.23
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

The expanding array of HIV broadly neutralizing antibodies.
McCoy LE. (2018)
3 more

6 mentions without citation

Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface.
Rujas et al. (2016)
5 more