› 5gg5

X-ray diffraction
1.64Å resolution

Crystal structure of Mycobacterium smegmatis MutT1

Released:
DOI: 10.2210/pdb5gg5/pdb
PDB entry 5gg5 coloured by chain, front view.
PDB entry 5gg5 coloured by chain, side view.
PDB entry 5gg5 coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Biochemical and structural studies of Mycobacterium smegmatis MutT1, a sanitization enzyme with unusual modes of association.
Arif SM, Patil AG, Varshney U, Vijayan M
Acta Crystallogr D Struct Biol 73 349-364 (2017)
PMID: 28375146

Function and Biology Details

Reactions catalysed: 
8-oxo-GDP + H(2)O = 8-oxo-GMP + phosphate
P(1),P(4)-bis(5'-adenosyl)tetraphosphate + H(2)O = ATP + AMP
8-oxo-GTP + H(2)O = 8-oxo-GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxo-(d)GTP phosphatase Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 38.15 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QUZ2 (Residues: 1-322; Coverage: 100%)
Gene names: MSMEG_2390, MSMEI_2330, mutT1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 11 x SO4
Ligand EDO 24 x EDO
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 37.45Å b: 87.66Å c: 101.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Guardians of the mycobacterial genome: A review on DNA repair systems in Mycobacterium tuberculosis.
Singh A. (2017)
4 other articles