› 5fkl

X-ray diffraction
1.9Å resolution

TetR(D) H100A mutant in complex with anhydrotetracycline and magnesium

Released:
DOI: 10.2210/pdb5fkl/pdb
PDB entry 5fkl coloured by chain, front view.
PDB entry 5fkl coloured by chain, side view.
PDB entry 5fkl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Modular organisation of inducer recognition and allostery in the tetracycline repressor.
Werten S, Schneider J, Palm GJ, Hinrichs W
FEBS J 283 2102-14 (2016)
PMID: 27028290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0ACT4 (Residues: 3-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand TDC 1 x TDC
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: I4122
Unit cell:
a: 66.557Å b: 66.557Å c: 179.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.265
Expression system: Escherichia coli K-12

Quick links

Citations

11 citation in other articles

Evolution-guided engineering of small-molecule biosensors.
Snoek et al. (2020)
10 more

1 mention without citation

Modular organisation of inducer recognition and allostery in the tetracycline repressor.
Werten et al. (2016)