› 5ffo

X-ray diffraction
3.49Å resolution

Integrin alpha V beta 6 in complex with pro-TGF-beta

Released:
DOI: 10.2210/pdb5ffo/pdb
PDB entry 5ffo coloured by chain, front view.
PDB entry 5ffo coloured by chain, side view.
PDB entry 5ffo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Force interacts with macromolecular structure in activation of TGF-β.
Dong X, Zhao B, Iacob RE, Zhu J, Koksal AC, Lu C, Engen JR, Springer TA
Nature 542 55-59 (2017)
PMID: 28117447

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-134064 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (10 distinct):
Integrin alpha-V heavy chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 601 amino acids
Theoretical weight: 65.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P06756 (Residues: 31-429, 430-627; Coverage: 59%)
Gene names: ITGAV, MSK8, VNRA, VTNR
Sequence domains:
Structure domains:
Integrin beta-6 Chains: B, F
Molecule details ›
Chains: B, F
Length: 257 amino acids
Theoretical weight: 28.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P18564 (Residues: 128-378; Coverage: 33%)
Gene name: ITGB6
Sequence domains: Integrin beta chain VWA domain
Structure domains: von Willebrand factor, type A domain
Transforming growth factor beta-1 proprotein Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 363 amino acids
Theoretical weight: 41.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01137 (Residues: 34-390; Coverage: 99%)
Gene names: TGFB, TGFB1
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand CA 8 x CA
Ligand NAG 4 x NAG
Ligand MN 6 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 81.44Å b: 91.44Å c: 131.03Å
α: 89.98° β: 86.25° γ: 89.85°
R-values:
R R work R free
0.226 0.224 0.277
Expression system: Homo sapiens

Quick links

Citations

53 review citations

Transforming growth factor-β1 in regulatory T cell biology.
Moreau et al. (2022)
52 more

14 mentions without citation

Force interacts with macromolecular structure in activation of TGF-β.
Dong et al. (2017)
13 more