› 5ewi

X-ray diffraction
1.6Å resolution

Crystal Structure of the Human Fab VRC38.01, an HIV-1 V1V2-Directed Neutralizing Antibody Isolated from Donor N90

Released:
DOI: 10.2210/pdb5ewi/pdb
PDB entry 5ewi coloured by chain, front view.
PDB entry 5ewi coloured by chain, side view.
PDB entry 5ewi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop.
Cale EM, Gorman J, Radakovich NA, Crooks ET, Osawa K, Tong T, Li J, Nagarajan R, Ozorowski G, Ambrozak DR, Asokan M, Bailer RT, Bennici AK, Chen X, Doria-Rose NA, Druz A, Feng Y, Joyce MG, Louder MK, O'Dell S, Oliver C, Pancera M, Connors M, Hope TJ, Kepler TB, Wyatt RT, Ward AB, Georgiev IS, Kwong PD, Mascola JR, Binley JM
Immunity 46 777-791.e10 (2017)
PMID: 28514685

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134636 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q6N089 (Residues: 20-117, 118-247; Coverage: 50%)
Gene name: DKFZp686P15220
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chain: L
Molecule details ›
Chain: L
Length: 220 amino acids
Theoretical weight: 24.12 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01834 (Residues: 1-107; Coverage: 100%)
Gene name: IGKC
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Unit cell:
a: 62.353Å b: 84.775Å c: 90.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.202
Expression system: Not provided

Quick links

Citations

12 review citations

Recent progress in broadly neutralizing antibodies to HIV.
Sok et al. (2018)
11 more

1 mention without citation

Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop.
Cale et al. (2017)