› 5eiu

X-ray diffraction
1.91Å resolution

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine--tRNA ligase; Tripartite motif-containing protein 5 Chains: A, D
Molecule details ›
Chains: A, D
Length: 141 amino acids
Theoretical weight: 16.47 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0PF16 (Residues: 89-159, 226-265; Coverage: 22%)
  • Canonical: P34945 (Residues: 49-78; Coverage: 7%)
Gene names: TRIM5, TT_C0520, serS
Sequence domains: B-box zinc finger

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 72.662Å b: 41.488Å c: 111.301Å
α: 90° β: 99.06° γ: 90°
R-values:
R R work R free
0.185 0.183 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Restriction of HIV-1 and other retroviruses by TRIM5.
Ganser-Pornillos et al. (2019)
14 more

3 mentions without citation

Conserved structural and functional aspects of the tripartite motif gene family point towards therapeutic applications in multiple diseases.
Gushchina et al. (2018)
2 more