› 5eba

X-ray diffraction
2.3Å resolution

Crystal structure of aromatic mutant (Y343A) of an alkali thermostable GH10 xylanase from Bacillus sp. NG-27

Released:
DOI: 10.2210/pdb5eba/pdb
PDB entry 5eba coloured by chain, front view.
PDB entry 5eba coloured by chain, side view.
PDB entry 5eba coloured by chain, top view.
Source organism: Bacillus sp. NG-27
Entry authors: Mahanta P, Bhardwaj A, Reddy VS, Ramakumar S

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128261 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 41.02 KDa
Source organism: Bacillus sp. NG-27
Expression system: Escherichia coli
UniProt:
  • Canonical: O30700 (Residues: 52-405; Coverage: 94%)
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 73.86Å b: 80.11Å c: 69.21Å
α: 90° β: 111.19° γ: 90°
R-values:
R R work R free
0.181 0.178 0.235
Expression system: Escherichia coli

Quick links