› 5e7z

X-ray diffraction
2.4Å resolution

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Mycobacterium tuberculosis in complex with D/L-tryptophan and D-phenylalanine

Released:
DOI: 10.2210/pdb5e7z/pdb
PDB entry 5e7z coloured by chain, front view.
PDB entry 5e7z coloured by chain, side view.
PDB entry 5e7z coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids.
Reichau S, Blackmore NJ, Jiao W, Parker EJ
PLoS One 11 e0152723 (2016)
PMID: 27128682

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase AroG Chains: A, B
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 50.83 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: O53512 (Residues: 1-462; Coverage: 100%)
Gene names: Rv2178c, aroG
Sequence domains: Class-II DAHP synthetase family

Ligands and Environments

6 bound ligands:
Ligand SO4 4 x SO4
Ligand MN 2 x MN
Ligand DTR 2 x DTR
Ligand TRP 2 x TRP
Ligand DPN 2 x DPN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P3221
Unit cell:
a: 206.357Å b: 206.357Å c: 66.941Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.138 0.137 0.161
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent advancements in the development of anti-tuberculosis drugs.
Chetty et al. (2017)
1 more

1 mention without citation

Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids.
Reichau et al. (2016)