› 5e0y

X-ray diffraction
2Å resolution

Crystal Structure of PASTA Domain 4 of Mycobacterium tuberculosis Protein Kinase B

Released:
DOI: 10.2210/pdb5e0y/pdb
PDB entry 5e0y coloured by chain, front view.
PDB entry 5e0y coloured by chain, side view.
PDB entry 5e0y coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site.
Prigozhin DM, Papavinasasundaram KG, Baer CE, Murphy KC, Moskaleva A, Chen TY, Alber T, Sassetti CM
J Biol Chem 291 22961-22969 (2016)
PMID: 27601474

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknB Chain: A
Molecule details ›
Chain: A
Length: 72 amino acids
Theoretical weight: 7.79 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WI81 (Residues: 558-626; Coverage: 11%)
Gene names: MTCY10H4.14c, Rv0014c, pknB
Sequence domains: PASTA domain
Structure domains: Trypsin Inhibitor V; Chain A

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6522
Unit cell:
a: 41.658Å b: 41.658Å c: 122.517Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.215 0.264
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Do Shoot the Messenger: PASTA Kinases as Virulence Determinants and Antibiotic Targets.
Pensinger et al. (2018)
2 more

1 mention without citation

Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site.
Prigozhin et al. (2016)