› 5dj5

X-ray diffraction
2.4Å resolution

Crystal structure of rice DWARF14 in complex with synthetic strigolactone GR24

Released:
DOI: 10.2210/pdb5dj5/pdb
PDB entry 5dj5 coloured by chain, front view.
PDB entry 5dj5 coloured by chain, side view.
PDB entry 5dj5 coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3.
Zhao LH, Zhou XE, Yi W, Wu Z, Liu Y, Kang Y, Hou L, de Waal PW, Li S, Jiang Y, Scaffidi A, Flematti GR, Smith SM, Lam VQ, Griffin PR, Wang Y, Li J, Melcher K, Xu HE
Cell Res 25 1219-36 (2015)
PMID: 26470846

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strigolactone esterase D14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.25 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q10QA5 (Residues: 52-318; Coverage: 84%)
Gene names: D14, D88, HTD2, LOC_Os03g10620, Os03g0203200
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand GR2 1 x GR2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 48.4Å b: 87.88Å c: 118.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Strigolactone Signaling and Evolution.
Waters et al. (2017)
26 more

17 mentions without citation

Unveiling the functional diversity of the alpha/beta hydrolase superfamily in the plant kingdom.
Mindrebo et al. (2016)
16 more