› 5dhl

X-ray diffraction
2.67Å resolution

Crystal structure of Toxin, mutant N197W

Released:
DOI: 10.2210/pdb5dhl/pdb
PDB entry 5dhl coloured by chain, front view.
PDB entry 5dhl coloured by chain, side view.
PDB entry 5dhl coloured by chain, top view.
Source organism: Clostridium perfringens str. 13
Entry authors: Parker MW, Gorman MA, Lawrence SL

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Perfringolysin O Chains: A, B
Molecule details ›
Chains: A, B
Length: 472 amino acids
Theoretical weight: 52.77 KDa
Source organism: Clostridium perfringens str. 13
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2E9 (Residues: 29-500; Coverage: 100%)
Gene names: CPE0163, pfo, pfoA, pfoR
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 161.629Å b: 212.398Å c: 46.958Å
α: 90° β: 97.17° γ: 90°
R-values:
R R work R free
0.193 0.191 0.239
Expression system: Escherichia coli

Quick links