› 5ddk

X-ray diffraction
2.13Å resolution

Succinyl-CoA:acetate CoA-transferase (AarCH6-N347A) in complex with CoA

Released:
DOI: 10.2210/pdb5ddk/pdb
PDB entry 5ddk coloured by chain, front view.
PDB entry 5ddk coloured by chain, side view.
PDB entry 5ddk coloured by chain, top view.
Source organism: Acetobacter aceti 1023
Primary publication:
Functional Dissection of the Bipartite Active Site of the Class I Coenzyme A (CoA)-Transferase Succinyl-CoA:Acetate CoA-Transferase.
Murphy JR, Mullins EA, Kappock TJ
Front Chem 4 23 (2016)
PMID: 27242998

Function and Biology Details

Reaction catalysed: 
Succinyl-CoA + acetate = acetyl-CoA + succinate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Succinyl-CoA:acetate CoA-transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 514 amino acids
Theoretical weight: 56 KDa
Source organism: Acetobacter aceti 1023
Expression system: Escherichia coli
UniProt:
  • Canonical: B3EY95 (Residues: 1-505; Coverage: 100%)
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand COA 2 x COA
Ligand IMD 3 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 67.103Å b: 109.775Å c: 120.057Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.192
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

IpdAB, a virulence factor in Mycobacterium tuberculosis, is a cholesterol ring-cleaving hydrolase.
Crowe et al. (2018)

2 mentions without citation

Thioesterase enzyme families: Functions, structures, and mechanisms.
Caswell et al. (2022)
1 more