› 5d8i

X-ray diffraction
2.05Å resolution

Engineering the Species-Specificity of an Inhibitory Antibody Targeting a Modulator of Tumor Stroma

Released:
DOI: 10.2210/pdb5d8i/pdb
PDB entry 5d8i coloured by chain, front view.
PDB entry 5d8i coloured by chain, side view.
PDB entry 5d8i coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Overcoming a species-specificity barrier in development of an inhibitory antibody targeting a modulator of tumor stroma.
Grossman I, Ilani T, Fleishman SJ, Fass D
Protein Eng Des Sel 29 135-47 (2016)
PMID: 26819240

Function and Biology Details

Reaction catalysed: 
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfhydryl oxidase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 26.59 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8BND5 (Residues: 36-275; Coverage: 34%)
Gene names: Qscn6, Qsox1, Sox
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 42.48Å b: 116.375Å c: 50.023Å
α: 90° β: 103.09° γ: 90°
R-values:
R R work R free
0.204 0.201 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Antibody Structure and Function: The Basis for Engineering Therapeutics.
Chiu et al. (2019)
2 more

1 mention without citation

Overcoming a species-specificity barrier in development of an inhibitory antibody targeting a modulator of tumor stroma.
Grossman et al. (2016)