5d5x

X-ray diffraction
2.4Å resolution

Crystal structure of Chaetomium thermophilum Skn7 with SSRE DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-118542 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor SKN7 Chains: B, E
Molecule details ›
Chains: B, E
Length: 104 amino acids
Theoretical weight: 12.62 KDa
Source organism: Chaetomium thermophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0SB31 (Residues: 40-143; Coverage: 14%)
Gene names: CTHT_0048700, SKN7
Sequence domains: HSF-type DNA-binding
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
SSRE DNA strand 1 Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
SSRE DNA strand 2 Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: C2
Unit cell:
a: 73.733Å b: 43.328Å c: 88.78Å
α: 90° β: 92.78° γ: 90°
R-values:
R R work R free
0.231 0.228 0.289
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided