› 5d5e

X-ray diffraction
2.41Å resolution

In meso in situ serial X-ray crystallography structure of insulin by sulfur-SAD at 100 K

Released:
DOI: 10.2210/pdb5d5e/pdb
PDB entry 5d5e coloured by chain, front view.
PDB entry 5d5e coloured by chain, side view.
PDB entry 5d5e coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Huang CY, Olieric V, Ma P, Howe N, Vogeley L, Liu X, Warshamanage R, Weinert T, Panepucci E, Kobilka B, Diederichs K, Wang M, Caffrey M
Acta Crystallogr D Struct Biol 72 93-112 (2016)
PMID: 26894538

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134237 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand PE5 1 x PE5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I213
Unit cell:
a: 78.36Å b: 78.36Å c: 78.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.22

Quick links

Citations

4 review citations

Changes in Membrane Protein Structural Biology.
Birch et al. (2020)
3 more

1 mention without citation

In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Huang et al. (2016)