› 5czz

X-ray diffraction
2.6Å resolution

Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGAAT PAM)

Released:
DOI: 10.2210/pdb5czz/pdb
PDB entry 5czz coloured by chain, front view.
PDB entry 5czz coloured by chain, side view.
PDB entry 5czz coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal Structure of Staphylococcus aureus Cas9.
Nishimasu H, Cong L, Yan WX, Ran FA, Zetsche B, Li Y, Kurabayashi A, Ishitani R, Zhang F, Nureki O
Cell 162 1113-26 (2015)
PMID: 26317473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118522 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 1056 amino acids
Theoretical weight: 124.37 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: J7RUA5 (Residues: 1-1053; Coverage: 100%)
Gene name: cas9
Sequence domains:
DNA (28-MER) Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.47 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
DNA (5'-D(*TP*TP*GP*AP*AP*TP*AP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
RNA (73-MER) Chain: B
Molecule details ›
Chain: B
Length: 73 nucleotides
Theoretical weight: 23.53 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NA 5 x NA
Ligand PO4 1 x PO4
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 67.585Å b: 345.584Å c: 98.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.241
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

79 review citations

CRISPR-Cas9 Structures and Mechanisms.
Jiang et al. (2017)
78 more

26 mentions without citation

Optimization of genome editing through CRISPR-Cas9 engineering.
Zhang et al. (2016)
25 more