› 5ckf

X-ray diffraction
2.8Å resolution

E. coli MazF E24A form I

Released:
DOI: 10.2210/pdb5ckf/pdb
PDB entry 5ckf coloured by chain, front view.
PDB entry 5ckf coloured by chain, side view.
PDB entry 5ckf coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Substrate Recognition and Activity Regulation of the Escherichia coli mRNA Endonuclease MazF.
Zorzini V, Mernik A, Lah J, Sterckx YG, De Jonge N, Garcia-Pino A, De Greve H, Versées W, Loris R
J Biol Chem 291 10950-60 (2016)
PMID: 27026704

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease toxin MazF Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE70 (Residues: 1-111; Coverage: 100%)
Gene names: JW2753, b2782, chpA, chpAK, mazF
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 52.816Å b: 52.816Å c: 191.398Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.236 0.3
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure, Biology, and Therapeutic Application of Toxin-Antitoxin Systems in Pathogenic Bacteria.
Lee et al. (2016)
4 more

1 mention without citation

Substrate Recognition and Activity Regulation of the Escherichia coli mRNA Endonuclease MazF.
Zorzini et al. (2016)