› 5cfv

X-ray diffraction
1.8Å resolution

Fusion of Maltose-binding Protein and PilA from Acinetobacter nosocomialis M2

Released:
DOI: 10.2210/pdb5cfv/pdb
PDB entry 5cfv coloured by chain, front view.
PDB entry 5cfv coloured by chain, side view.
PDB entry 5cfv coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Diversity in the Type IV Pili of Multidrug-resistant Acinetobacter.
Piepenbrink KH, Lillehoj E, Harding CM, Labonte JW, Zuo X, Rapp CA, Munson RS, Goldblum SE, Feldman MF, Gray JJ, Sundberg EJ
J Biol Chem 291 22924-22935 (2016)
PMID: 27634041

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 53.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand MPD 1 x MPD
Ligand GOL 3 x GOL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 173.883Å b: 55.334Å c: 49.67Å
α: 90° β: 91.52° γ: 90°
R-values:
R R work R free
0.182 0.182 0.228
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Uncovering the mechanisms of Acinetobacter baumannii virulence.
Harding et al. (2018)
6 more

1 mention without citation

Structural Diversity in the Type IV Pili of Multidrug-resistant Acinetobacter.
Piepenbrink et al. (2016)