› 5c2j

X-ray diffraction
2.5Å resolution

Complex structure of the GAP domain of MgcRacGAP and Cdc42

Released:
DOI: 10.2210/pdb5c2j/pdb
PDB entry 5c2j coloured by chain, front view.
PDB entry 5c2j coloured by chain, side view.
PDB entry 5c2j coloured by chain, top view.
Source organisms:
Entry authors: Murayama K, Kato-Murayama M, Hosaka T, Kitamura T, Yokoyama S, Shirouzu M

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158092 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rac GTPase-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H0H5 (Residues: 346-546; Coverage: 32%)
Gene names: KIAA1478, MGCRACGAP, RACGAP1
Sequence domains: RhoGAP domain
Structure domains: Rho GTPase activation protein
Cell division control protein 42 homolog Chain: B
Molecule details ›
Chain: B
Length: 198 amino acids
Theoretical weight: 21.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60766 (Residues: 1-191; Coverage: 100%)
Gene name: Cdc42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand AF3 1 x AF3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 43.324Å b: 74.237Å c: 55.423Å
α: 90° β: 96.67° γ: 90°
R-values:
R R work R free
0.198 0.198 0.263
Expression system: Escherichia coli

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