› 5bpl

X-ray diffraction
1.93Å resolution

Crystal structure of ADP and Pi bound human Hsp70 NBD mutant R272K.

Released:
DOI: 10.2210/pdb5bpl/pdb
PDB entry 5bpl coloured by chain, front view.
PDB entry 5bpl coloured by chain, side view.
PDB entry 5bpl coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Narayanan D, Engh RA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock 70 kDa protein 1A Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 42.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DMV8 (Residues: 1-388; Coverage: 61%)
Gene names: HSP72, HSPA1, HSPA1A, HSX70
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ADP 1 x ADP
Ligand PO4 3 x PO4
Ligand MG 1 x MG
Ligand NA 2 x NA
Ligand EPE 1 x EPE
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 46.037Å b: 63.327Å c: 143.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.183 0.239
Expression system: Escherichia coli BL21(DE3)

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