› 5aza

X-ray diffraction
2.08Å resolution

Crystal structure of MBP-sAglB fusion protein with a 20-residue spacer in the connector helix

Released:
DOI: 10.2210/pdb5aza/pdb
PDB entry 5aza coloured by chain, front view.
PDB entry 5aza coloured by chain, side view.
PDB entry 5aza coloured by chain, top view.
Source organisms:
Primary publication:
Rational design of crystal contact-free space in protein crystals for analyzing spatial distribution of motions within protein molecules.
Matsuoka R, Shimada A, Komuro Y, Sugita Y, Kohda D
Protein Sci 25 754-68 (2016)
PMID: 26694222

Function and Biology Details

Reaction catalysed: 
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125161 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; dolichyl-phosphooligosaccharide-protein glycotransferase Chain: A
Molecule details ›
Chain: A
Length: 872 amino acids
Theoretical weight: 97.12 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-394; Coverage: 100%)
  • Canonical: I6V0B8 (Residues: 491-967; Coverage: 49%)
Gene names: JW3994, PFC_07420, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P212121
Unit cell:
a: 66.468Å b: 100.467Å c: 140.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.248
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Role of Computational Methods in Going beyond X-ray Crystallography to Explore Protein Structure and Dynamics.
Srivastava et al. (2018)
6 other articles

10 mentions without citation

Targeting FBPase is an emerging novel approach for cancer therapy.
Liu et al. (2018)
9 more