› 5ayh

X-ray diffraction
3.01Å resolution

Structure of the entire dynein stalk region

Released:
DOI: 10.2210/pdb5ayh/pdb
PDB entry 5ayh coloured by chain, front view.
PDB entry 5ayh coloured by chain, side view.
PDB entry 5ayh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural Change in the Dynein Stalk Region Associated with Two Different Affinities for the Microtubule.
Nishikawa Y, Inatomi M, Iwasaki H, Kurisu G
J Mol Biol 428 1886-96 (2016)
PMID: 26585405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytoplasmic dynein 1 heavy chain 1 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JHU4 (Residues: 3206-3475; Coverage: 6%)
Gene names: Dhc1, Dnch1, Dnchc1, Dyhc, Dync1h1
Sequence domains: Microtubule-binding stalk of dynein motor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 103.325Å b: 103.325Å c: 69.709Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.259 0.255 0.295
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Coiled-coils: The long and short of it.
Truebestein et al. (2016)
1 more

2 mentions without citation

Structural determinants of Rab11 activation by the guanine nucleotide exchange factor SH3BP5.
Jenkins et al. (2018)
1 more