› 4zoh

X-ray diffraction
2.2Å resolution

Crystal structure of glyceraldehyde oxidoreductase

Released:
DOI: 10.2210/pdb4zoh/pdb
PDB entry 4zoh coloured by chain, front view.
PDB entry 4zoh coloured by chain, side view.
PDB entry 4zoh coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii str. 7
Primary publication:
Archaeal Mo-Containing Glyceraldehyde Oxidoreductase Isozymes Exhibit Diverse Substrate Specificities through Unique Subunit Assemblies.
Wakagi T, Nishimasu H, Miyake M, Fushinobu S
PLoS One 11 e0147333 (2016)
PMID: 26808202

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-188788 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 706 amino acids
Theoretical weight: 77.85 KDa
Source organism: Sulfurisphaera tokodaii str. 7
UniProt:
  • Canonical: Q96Y29 (Residues: 1-706; Coverage: 100%)
Gene names: ST2339, STK_23390
Sequence domains:
Structure domains:
FAD-binding PCMH-type domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 278 amino acids
Theoretical weight: 30.75 KDa
Source organism: Sulfurisphaera tokodaii str. 7
UniProt:
  • Canonical: Q974U9 (Residues: 1-278; Coverage: 100%)
Gene names: ST0562, STK_05620
Sequence domains:
Structure domains:
2Fe-2S ferredoxin-type domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 168 amino acids
Theoretical weight: 18.64 KDa
Source organism: Sulfurisphaera tokodaii str. 7
UniProt:
  • Canonical: Q974V0 (Residues: 1-168; Coverage: 100%)
Gene names: ST0561, STK_05610
Sequence domains:
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand MCN 1 x MCN
Ligand MO 1 x MO
Ligand 1PE 1 x 1PE
Ligand PEG 4 x PEG
Ligand FAD 1 x FAD
Ligand PG4 1 x PG4
Ligand ACY 1 x ACY
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P6522
Unit cell:
a: 143.431Å b: 143.431Å c: 235.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.242

Quick links

Citations

2 citation in other articles

A systems biology approach reveals major metabolic changes in the thermoacidophilic archaeon Sulfolobus solfataricus in response to the carbon source L-fucose versus D-glucose.
Wolf et al. (2016)
1 more

1 mention without citation

Archaeal Mo-Containing Glyceraldehyde Oxidoreductase Isozymes Exhibit Diverse Substrate Specificities through Unique Subunit Assemblies.
Wakagi et al. (2016)