› 4zir

X-ray diffraction
3Å resolution

Crystal structure of EcfAA' heterodimer bound to AMPPNP

Released:
DOI: 10.2210/pdb4zir/pdb
PDB entry 4zir coloured by chain, front view.
PDB entry 4zir coloured by chain, side view.
PDB entry 4zir coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism.
Karpowich NK, Song JM, Cocco N, Wang DN
Nat Struct Mol Biol 22 565-71 (2015)
PMID: 26052893

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194473 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Energy-coupling factor transporter ATP-binding protein EcfA2 Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 30.33 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WY65 (Residues: 2-266; Coverage: 100%)
Gene names: TM_0222, ecfA, ecfA', ecfA2
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Energy-coupling factor transporter ATP-binding protein EcfA1 Chain: B
Molecule details ›
Chain: B
Length: 268 amino acids
Theoretical weight: 29.86 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X1Z1 (Residues: 2-259; Coverage: 100%)
Gene names: TM_1663, cbiO, ecfA', ecfA1
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CL 3 x CL
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P42212
Unit cell:
a: 107.275Å b: 107.275Å c: 96.758Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.192 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Overlapping riboflavin supply pathways in bacteria.
García-Angulo VA. (2017)
5 more

3 mentions without citation

ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism.
Karpowich et al. (2015)
2 more