› 4zfo

X-ray diffraction
1.9Å resolution

J22.9-xi: chimeric mouse/human antibody against human BCMA (CD269)

Released:
DOI: 10.2210/pdb4zfo/pdb
PDB entry 4zfo coloured by chain, front view.
PDB entry 4zfo coloured by chain, side view.
PDB entry 4zfo coloured by chain, top view.
Source organisms:
Primary publication:
Potent anti-tumor response by targeting B cell maturation antigen (BCMA) in a mouse model of multiple myeloma.
Oden F, Marino SF, Brand J, Scheu S, Kriegel C, Olal D, Takvorian A, Westermann J, Yilmaz B, Hinz M, Daumke O, Höpken UE, Müller G, Lipp M
Mol Oncol 9 1348-58 (2015)
PMID: 25953704

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211892 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tumor necrosis factor receptor superfamily member 17 Chains: F, K
Molecule details ›
Chains: F, K
Length: 54 amino acids
Theoretical weight: 5.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02223 (Residues: 1-54; Coverage: 29%)
Gene names: BCM, BCMA, TNFRSF17
Sequence domains: BCMA, TALL-1 binding
J22.9-xi Fab, Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.54 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
J22.9-xi Fab, Heavy Chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 221 amino acids
Theoretical weight: 23.64 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
J22.9-xi Fab, Light Chain Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CU 3 x CU
Ligand BTB 2 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 72.801Å b: 110.139Å c: 137.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.209
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

Quick links

Citations

5 review citations

Monoclonal antibody therapy in multiple myeloma.
Touzeau et al. (2017)
4 more

7 mentions without citation

CAR T Cells with Enhanced Sensitivity to B Cell Maturation Antigen for the Targeting of B Cell Non-Hodgkin's Lymphoma and Multiple Myeloma.
Bluhm et al. (2018)
6 more