› 4z31

X-ray diffraction
2.5Å resolution

Crystal structure of the RC3H2 ROQ domain in complex with stem-loop and double-stranded forms of RNA

Released:
DOI: 10.2210/pdb4z31/pdb
PDB entry 4z31 coloured by chain, front view.
PDB entry 4z31 coloured by chain, side view.
PDB entry 4z31 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Zhang Q, Fan L, Hou F, Dong A, Wang YX, Tong Y
Sci Rep 5 15660 (2015)
PMID: 26489670

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190745 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Roquin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 35.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HBD1 (Residues: 87-404; Coverage: 27%)
Gene names: MNAB, RC3H2, RNF164
Sequence domains:
RNA (5'-R(*A)-D(P*UP*GP*UP*UP*CP*UP*GP*UP*GP*AP*AP*CP*AP*C)-3') Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 15 nucleotides
Theoretical weight: 4.75 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand UNX 2 x UNX
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.299Å b: 174.734Å c: 61.29Å
α: 90° β: 114.12° γ: 90°
R-values:
R R work R free
0.245 0.245 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Ubiquitin signaling in immune responses.
Hu et al. (2016)
6 more