4ytx

X-ray diffraction
3.2Å resolution

Crystal structure of Ups1-Mdm35 complex with PA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-129910 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial distribution and morphology protein 35 Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 81 amino acids
Theoretical weight: 9.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: O60200 (Residues: 1-81; Coverage: 94%)
Gene names: MDM35, YKL053C-A
Sequence domains: Uncharacterised protein family (UPF0203)
Protein UPS1, mitochondrial Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 184 amino acids
Theoretical weight: 21.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05776 (Residues: 1-170; Coverage: 97%)
Gene names: UPS1, YLR193C
Sequence domains: PRELI-like family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 208.642Å b: 154.67Å c: 99.012Å
α: 90° β: 104.42° γ: 90°
R-values:
R R work R free
0.251 0.251 0.3
Expression system: Escherichia coli