› 4ydh

X-ray diffraction
3.8Å resolution

The structure of human FMNL1 N-terminal domains bound to Cdc42

Released:
DOI: 10.2210/pdb4ydh/pdb
PDB entry 4ydh coloured by chain, front view.
PDB entry 4ydh coloured by chain, side view.
PDB entry 4ydh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of FMNL2-Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation.
Kühn S, Erdmann C, Kage F, Block J, Schwenkmezger L, Steffen A, Rottner K, Geyer M
Nat Commun 6 7088 (2015)
PMID: 25963737

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131938 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Formin-like protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 433 amino acids
Theoretical weight: 49.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95466 (Residues: 1-458; Coverage: 39%)
Gene names: C17orf1, C17orf1B, FMNL, FMNL1, FRL1
Sequence domains:
Cell division control protein 42 homolog Chains: B, D
Molecule details ›
Chains: B, D
Length: 181 amino acids
Theoretical weight: 20.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60953 (Residues: 1-179; Coverage: 94%)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 87.6Å b: 102.1Å c: 170.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.281
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Membrane curvature in cell biology: An integration of molecular mechanisms.
Jarsch et al. (2016)
10 more

3 mentions without citation

The structure of FMNL2-Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation.
Kühn et al. (2015)
2 more