› 4yb0

X-ray diffraction
2.12Å resolution

3',3'-cGAMP riboswitch bound with c-di-GMP

Released:
DOI: 10.2210/pdb4yb0/pdb
PDB entry 4yb0 coloured by chain, front view.
PDB entry 4yb0 coloured by chain, side view.
PDB entry 4yb0 coloured by chain, top view.
Source organism: Geobacter
Primary publication:
Structural basis for molecular discrimination by a 3',3'-cGAMP sensing riboswitch.
Ren A, Wang XC, Kellenberger CA, Rajashankar KR, Jones RA, Hammond MC, Patel DJ
Cell Rep 11 1-12 (2015)
PMID: 25818298

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195897 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (84-MER) Chains: A, R
Molecule details ›
Chains: A, R
Length: 83 nucleotides
Theoretical weight: 26.83 KDa
Source organism: Geobacter
Expression system: in vitro transcription vector pT7-Fluc(deltai)
Sequence domains: Cyclic di-GMP-I riboswitch

Ligands and Environments

4 bound ligands:
Ligand GDP 2 x GDP
Ligand C2E 2 x C2E
Ligand MG 5 x MG
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 174.829Å b: 44.901Å c: 68.21Å
α: 90° β: 103.33° γ: 90°
R-values:
R R work R free
0.23 0.228 0.262
Expression system: in vitro transcription vector pT7-Fluc(deltai)

Quick links

Citations

9 review citations

Maximising the potential of AKT inhibitors as anti-cancer treatments.
Brown et al. (2017)
8 more

7 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
6 more