› 4y4s

X-ray diffraction
1.75Å resolution

Crystal Structure of Y75A HasA dimer from Yersinia pseudotuberculosis

Released:
DOI: 10.2210/pdb4y4s/pdb
PDB entry 4y4s coloured by chain, front view.
PDB entry 4y4s coloured by chain, side view.
PDB entry 4y4s coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis IP 32953
Primary publication:
The crystal structure of heme acquisition system A from Yersinia pseudotuberculosis (HasAypt): Roles of the axial ligand Tyr75 and two distal arginines in heme binding.
Kanadani M, Sato T, Hino T, Nagano S, Ozaki S
J Inorg Biochem 151 26-33 (2015)
PMID: 26210321

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular heme acquisition hemophore HasA Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 23.56 KDa
Source organism: Yersinia pseudotuberculosis IP 32953
Expression system: Escherichia coli
UniProt:
  • Canonical: Q66G68 (Residues: 1-205; Coverage: 100%)
Gene names: HasA, YPTB0114
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P43212
Unit cell:
a: 52.76Å b: 52.76Å c: 140.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.24
Expression system: Escherichia coli

Quick links