› 4xz6

X-ray diffraction
2.2Å resolution

TmoX in complex with TMAO

Released:
DOI: 10.2210/pdb4xz6/pdb
PDB entry 4xz6 coloured by chain, front view.
PDB entry 4xz6 coloured by chain, side view.
PDB entry 4xz6 coloured by chain, top view.
Source organism: Ruegeria pomeroyi DSS-3
Primary publication:
Mechanistic Insight into Trimethylamine N-Oxide Recognition by the Marine Bacterium Ruegeria pomeroyi DSS-3.
Li CY, Chen XL, Shao X, Wei TD, Wang P, Xie BB, Qin QL, Zhang XY, Su HN, Song XY, Shi M, Zhou BC, Zhang YZ
J Bacteriol 197 3378-87 (2015)
PMID: 26283766

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trimethylamine N-oxide-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 37.33 KDa
Source organism: Ruegeria pomeroyi DSS-3
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LT66 (Residues: 1-333; Coverage: 100%)
Gene names: SPO1548, tmoX
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system

Ligands and Environments

4 bound ligands:
Ligand TMO 2 x TMO
Ligand CA 2 x CA
Ligand GOL 2 x GOL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 29.624Å b: 134.979Å c: 58.366Å
α: 90° β: 90.15° γ: 90°
R-values:
R R work R free
0.177 0.174 0.24
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Microbial modulation of cardiovascular disease.
Brown et al. (2018)
5 other articles

3 mentions without citation

Mechanistic Insight into Trimethylamine N-Oxide Recognition by the Marine Bacterium Ruegeria pomeroyi DSS-3.
Li et al. (2015)
2 more