› 4xwg

X-ray diffraction
2.65Å resolution

Crystal Structure of LCAT (C31Y) in complex with Fab1

Released:
DOI: 10.2210/pdb4xwg/pdb
PDB entry 4xwg coloured by chain, front view.
PDB entry 4xwg coloured by chain, side view.
PDB entry 4xwg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The high-resolution crystal structure of human LCAT.
Piper DE, Romanow WG, Gunawardane RN, Fordstrom P, Masterman S, Pan O, Thibault ST, Zhang R, Meininger D, Schwarz M, Wang Z, King C, Zhou M, Walker NP
J Lipid Res 56 1711-9 (2015)
PMID: 26195816

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208991 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab1 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab1 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 25.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Phosphatidylcholine-sterol acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 47.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04180 (Residues: 25-440; Coverage: 100%)
Gene name: LCAT
Sequence domains: Lecithin:cholesterol acyltransferase
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand ZN 16 x ZN
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: R3
Unit cell:
a: 168.59Å b: 168.59Å c: 93.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.176 0.251
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Lysosomal phospholipase A2.
Shayman et al. (2019)
3 more

10 mentions without citation

The high-resolution crystal structure of human LCAT.
Piper et al. (2015)
9 more