› 4xtl

X-ray diffraction
1.45Å resolution

Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3

Released:
DOI: 10.2210/pdb4xtl/pdb
PDB entry 4xtl coloured by chain, front view.
PDB entry 4xtl coloured by chain, side view.
PDB entry 4xtl coloured by chain, top view.
Source organism: Dokdonia eikasta
Primary publication:
Crystal structure of a light-driven sodium pump.
Gushchin I, Shevchenko V, Polovinkin V, Kovalev K, Alekseev A, Round E, Borshchevskiy V, Balandin T, Popov A, Gensch T, Fahlke C, Bamann C, Willbold D, Büldt G, Bamberg E, Gordeliy V
Nat Struct Mol Biol 22 390-5 (2015)
PMID: 25849142

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium pumping rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 32.88 KDa
Source organism: Dokdonia eikasta
Expression system: Escherichia coli
UniProt:
  • Canonical: N0DKS8 (Residues: 1-280; Coverage: 100%)
Gene name: NaR
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand LFA 34 x LFA
Ligand GOL 2 x GOL
1 modified residue:
Ligand LYR 1 x LYR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 40.18Å b: 80.84Å c: 232.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.216
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Silencing Neurons: Tools, Applications, and Experimental Constraints.
Wiegert et al. (2017)
13 more

4 mentions without citation

Structure and mechanisms of sodium-pumping KR2 rhodopsin.
Kovalev et al. (2019)
3 more