› 4xlv

X-ray diffraction
2.3Å resolution

Crystal structure of the activated insulin receptor tyrosine kinase dimer

Released:
DOI: 10.2210/pdb4xlv/pdb
PDB entry 4xlv coloured by chain, front view.
PDB entry 4xlv coloured by chain, side view.
PDB entry 4xlv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The insulin and IGF1 receptor kinase domains are functional dimers in the activated state.
Cabail MZ, Li S, Lemmon E, Bowen ME, Hubbard SR, Miller WT
Nat Commun 6 6406 (2015)
PMID: 25758790

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06213 (Residues: 983-1310; Coverage: 24%)
Gene name: INSR
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACP 1 x ACP
Ligand MG 2 x MG
1 modified residue:
Ligand PTR 4 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3221
Unit cell:
a: 66.982Å b: 66.982Å c: 136.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.204 0.266
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

Primary Cilia and Coordination of Receptor Tyrosine Kinase (RTK) and Transforming Growth Factor β (TGF-β) Signaling.
Christensen et al. (2017)
7 more

8 mentions without citation

More than the sum of the parts: Toward full-length receptor tyrosine kinase structures.
Diwanji et al. (2019)
7 more