› 4x3g

X-ray diffraction
2.34Å resolution

Crystal structure of SIAH1 SINA domain in complex with a USP19 peptide

Released:
DOI: 10.2210/pdb4x3g/pdb
PDB entry 4x3g coloured by chain, front view.
PDB entry 4x3g coloured by chain, side view.
PDB entry 4x3g coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Walker JR, Dong A, Zhang Q, Huang X, Li Y, Bountra C, Edwards AM, Arrowsmith CH, Tong Y, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131804 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SIAH1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 21.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IUQ4 (Residues: 91-282; Coverage: 68%)
Gene names: HUMSIAH, SIAH1
Sequence domains:
Structure domains:
Ubiquitin carboxyl-terminal hydrolase 19 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O94966 (Residues: 461-474; Coverage: 1%)
Gene names: KIAA0891, USP19, ZMYND9

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 41.343Å b: 88.092Å c: 59.59Å
α: 90° β: 103.24° γ: 90°
R-values:
R R work R free
0.223 0.222 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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