› 4x0l

X-ray diffraction
2.05Å resolution

Human haptoglobin-haemoglobin complex

Released:
DOI: 10.2210/pdb4x0l/pdb
PDB entry 4x0l coloured by chain, front view.
PDB entry 4x0l coloured by chain, side view.
PDB entry 4x0l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for ligand and innate immunity factor uptake by the trypanosome haptoglobin-haemoglobin receptor.
Lane-Serff H, MacGregor P, Lowe ED, Carrington M, Higgins MK
Elife 3 e05553 (2014)
PMID: 25497229

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-133187 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemoglobin subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chain: B
Molecule details ›
Chain: B
Length: 146 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins
Haptoglobin beta chain Chain: C
Molecule details ›
Chain: C
Length: 259 amino acids
Theoretical weight: 28.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00738 (Residues: 148-406; Coverage: 67%)
Gene name: HP
Sequence domains: Trypsin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
5 bound ligands:
Ligand HEM 2 x HEM
Ligand OXY 2 x OXY
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
Ligand CAC 1 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3121
Unit cell:
a: 96.599Å b: 96.599Å c: 132.771Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.224
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

5 review citations

Haptoglobin.
Andersen et al. (2017)
4 more

4 mentions without citation

Structure-function analyses reveal key features in Staphylococcus aureus IsdB-associated unfolding of the heme-binding pocket of human hemoglobin.
Bowden et al. (2018)
3 more