Function and Biology Details
Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-143268 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 328 amino acids
Theoretical weight: 37.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
- Canonical:
Q9WUP7 (Residues: 1-329; Coverage: 100%)
Sequence domains:
Proteasomal ubiquitin receptor ADRM1
Molecule details ›
Chain: B
Length: 102 amino acids
Theoretical weight: 10.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: UCH-binding domain
Length: 102 amino acids
Theoretical weight: 10.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: Q16186 (Residues: 285-386; Coverage: 25%)
Sequence domains: UCH-binding domain
Ubiquitin
Molecule details ›
Chain: C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Ubiquitin family
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Sequence domains: Ubiquitin family
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X29A
Spacegroup:
P212121
Expression system: Escherichia coli
