› 4wis

X-ray diffraction
3.3Å resolution

Crystal structure of the lipid scramblase nhTMEM16 in crystal form 1

Released:
DOI: 10.2210/pdb4wis/pdb
PDB entry 4wis coloured by chain, front view.
PDB entry 4wis coloured by chain, side view.
PDB entry 4wis coloured by chain, top view.
Source organism: Fusarium vanettenii 77-13-4
Primary publication:
X-ray structure of a calcium-activated TMEM16 lipid scramblase.
Brunner JD, Lim NK, Schenck S, Duerst A, Dutzler R
Nature 516 207-12 (2014)
PMID: 25383531

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasma membrane channel protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 735 amino acids
Theoretical weight: 83.2 KDa
Source organism: Fusarium vanettenii 77-13-4
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: C7Z7K1 (Residues: 1-735; Coverage: 100%)
Gene name: NECHADRAFT_66456
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 96.48Å b: 113.69Å c: 235.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.285
Expression system: Saccharomyces cerevisiae

Quick links

Citations

63 review citations

An Apoptotic 'Eat Me' Signal: Phosphatidylserine Exposure.
Segawa et al. (2015)
62 more

43 mentions without citation

Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.
Muller et al. (2019)
42 more