› 4v1l

X-ray diffraction
1.75Å resolution

High resolution structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1

Released:
DOI: 10.2210/pdb4v1l/pdb
PDB entry 4v1l coloured by chain, front view.
PDB entry 4v1l coloured by chain, side view.
PDB entry 4v1l coloured by chain, top view.
Source organism: Ruminococcus flavefaciens FD-1
Primary publication:
Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition.
Venditto I, Luis AS, Rydahl M, Schückel J, Fernandes VO, Vidal-Melgosa S, Bule P, Goyal A, Pires VM, Dourado CG, Ferreira LM, Coutinho PM, Henrissat B, Knox JP, Baslé A, Najmudin S, Gilbert HJ, Willats WG, Fontes CM
Proc Natl Acad Sci U S A 113 7136-41 (2016)
PMID: 27298375

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF5620 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 138 amino acids
Theoretical weight: 15.33 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli B
UniProt:
  • Canonical: A0A140UH31 (Residues: 1-138; Coverage: 100%)
Sequence domains: Domain of unknown function (DUF5620)

Ligands and Environments

4 bound ligands:
Ligand P6G 3 x P6G
Ligand GOL 3 x GOL
Ligand PGE 2 x PGE
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I212121
Unit cell:
a: 102.25Å b: 102.525Å c: 109.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.18
Expression system: Escherichia coli B

Quick links

Citations

8 review citations

Advances in molecular engineering of carbohydrate-binding modules.
Armenta et al. (2017)
7 more

1 mention without citation

Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition.
Venditto et al. (2016)