› 4v0j

X-ray diffraction
2.8Å resolution

The channel-block Ser202Glu, Thr104Lys double mutant of Stearoyl-ACP- Desaturase from Castor bean (Ricinus communis)

Released:
DOI: 10.2210/pdb4v0j/pdb
PDB entry 4v0j coloured by chain, front view.
PDB entry 4v0j coloured by chain, side view.
PDB entry 4v0j coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Half-of-the-Sites Reactivity of the Castor Δ9-18:0-Acyl Carrier Protein Desaturase.
Liu Q, Chai J, Moche M, Guy J, Lindqvist Y, Shanklin J
Plant Physiol 169 432-41 (2015)
PMID: 26224800

Function and Biology Details

Reaction catalysed: 
Stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O(2) + 2 H(+) = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 331 amino acids
Theoretical weight: 38.19 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P22337 (Residues: 66-396; Coverage: 84%)
Sequence domains: Fatty acid desaturase
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand FE2 12 x FE2
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P31
Unit cell:
a: 139.719Å b: 139.719Å c: 86.871Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.264
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Plasmalogens and Photooxidative Stress Signaling in Myxobacteria, and How it Unmasked CarF/TMEM189 as the Δ1'-Desaturase PEDS1 for Human Plasmalogen Biosynthesis.
Padmanabhan et al. (2022)
7 other articles