› 4ux9

X-ray diffraction
2.34Å resolution

Crystal structure of JNK1 bound to a MKK7 docking motif

Released:
DOI: 10.2210/pdb4ux9/pdb
PDB entry 4ux9 coloured by chain, front view.
PDB entry 4ux9 coloured by chain, side view.
PDB entry 4ux9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and dynamics of the MKK7-JNK signaling complex.
Kragelj J, Palencia A, Nanao MH, Maurin D, Bouvignies G, Blackledge M, Jensen MR
Proc Natl Acad Sci U S A 112 3409-14 (2015)
PMID: 25737554

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127071 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 364 amino acids
Theoretical weight: 42.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45983 (Residues: 1-364; Coverage: 85%)
Gene names: JNK1, MAPK8, PRKM8, SAPK1, SAPK1C
Sequence domains: Protein kinase domain
Structure domains:
Dual specificity mitogen-activated protein kinase kinase 7 Chains: F, G, H, I
Molecule details ›
Chains: F, G, H, I
Length: 12 amino acids
Theoretical weight: 1.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O14733 (Residues: 37-48; Coverage: 3%)
Gene names: JNKK2, MAP2K7, MEK7, MKK7, PRKMK7, SKK4

Ligands and Environments

2 bound ligands:
Ligand ANP 4 x ANP
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 108.666Å b: 180.159Å c: 101.144Å
α: 90° β: 110.3° γ: 90°
R-values:
R R work R free
0.202 0.2 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

JNK Signaling: Regulation and Functions Based on Complex Protein-Protein Partnerships.
Zeke et al. (2016)
11 more

8 mentions without citation

Bulbus Fritillariae Cirrhosae as a Respiratory Medicine: Is There a Potential Drug in the Treatment of COVID-19?
Quan et al. (2021)
7 more