› 4unv

X-ray diffraction
1.6Å resolution

Covalent dimer of lambda variable domains

Released:
DOI: 10.2210/pdb4unv/pdb
PDB entry 4unv coloured by chain, front view.
PDB entry 4unv coloured by chain, side view.
PDB entry 4unv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Formation of amyloid fibers by monomeric light chain variable domains.
Brumshtein B, Esswein SR, Landau M, Ryan CM, Whitelegge JP, Phillips ML, Cascio D, Sawaya MR, Eisenberg DS
J Biol Chem 289 27513-25 (2014)
PMID: 25138218

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin lambda variable 2-8 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 11.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01709 (Residues: 20-118; Coverage: 100%)
Gene name: IGLV2-8
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 37.417Å b: 60.212Å c: 79.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Screening methods for identifying pharmacological chaperones.
Shin et al. (2017)
1 more

1 mention without citation

Formation of amyloid fibers by monomeric light chain variable domains.
Brumshtein et al. (2014)