› 4uem

X-ray diffraction
2.82Å resolution

UCH-L5 in complex with the RPN13 DEUBAD domain

Released:
DOI: 10.2210/pdb4uem/pdb
PDB entry 4uem coloured by chain, front view.
PDB entry 4uem coloured by chain, side view.
PDB entry 4uem coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of UCH-L5 activation and inhibition by DEUBAD domains in RPN13 and INO80G.
Sahtoe DD, van Dijk WJ, El Oualid F, Ekkebus R, Ovaa H, Sixma TK
Mol Cell 57 887-900 (2015)
PMID: 25702870

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172453 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5K5 (Residues: 1-329; Coverage: 100%)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains:
Structure domains:
Proteasomal ubiquitin receptor ADRM1 Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 13.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16186 (Residues: 266-388; Coverage: 30%)
Gene names: ADRM1, GP110
Sequence domains: UCH-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 56.596Å b: 97.067Å c: 100.616Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Mechanisms of Deubiquitinase Specificity and Regulation.
Mevissen et al. (2017)
29 more

9 mentions without citation

Mechanisms orchestrating the enzymatic activity and cellular functions of deubiquitinases.
Estavoyer et al. (2022)
8 more