› 4u5r

X-ray diffraction
1.55Å resolution

Crystal structure of D106A mutant of RhCC (YP_702633.1) from Rhodococcus jostii RHA1 at 1.55 Angstrom

Released:
DOI: 10.2210/pdb4u5r/pdb
PDB entry 4u5r coloured by chain, front view.
PDB entry 4u5r coloured by chain, side view.
PDB entry 4u5r coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Primary publication:
Functional and structural characterization of an unusual cofactor-independent oxygenase.
Baas BJ, Poddar H, Geertsema EM, Rozeboom HJ, de Vries MP, Permentier HP, Thunnissen AM, Poelarends GJ
Biochemistry 54 1219-32 (2015)
PMID: 25565350

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tautomerase cis-CaaD-like domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 152 amino acids
Theoretical weight: 17.64 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0SDB1 (Residues: 2-146; Coverage: 99%)
Gene name: RHA1_ro02670
Sequence domains: Putative oxalocrotonate tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 99.795Å b: 57.784Å c: 92.026Å
α: 90° β: 90.41° γ: 90°
R-values:
R R work R free
0.16 0.16 0.175
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

New insight into cofactor-free oxygenation from combined experimental and computational approaches.
Bui et al. (2016)
5 other articles