› 4u3j

X-ray diffraction
2.81Å resolution

TOG2:alpha/beta-tubulin complex

Released:
DOI: 10.2210/pdb4u3j/pdb
PDB entry 4u3j coloured by chain, front view.
PDB entry 4u3j coloured by chain, side view.
PDB entry 4u3j coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A tethered delivery mechanism explains the catalytic action of a microtubule polymerase.
Ayaz P, Munyoki S, Geyer EA, Piedra FA, Vu ES, Bromberg R, Otwinowski Z, Grishin NV, Brautigam CA, Rice LM
Elife 3 e03069 (2014)
PMID: 25097237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135932 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1 chain Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.85 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P09733 (Residues: 1-447; Coverage: 100%)
Gene names: TUB1, YML085C
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 463 amino acids
Theoretical weight: 51.91 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P02557 (Residues: 1-457; Coverage: 100%)
Gene names: TUB2, YFL037W
Sequence domains:
Structure domains:
Protein STU2 Chain: C
Molecule details ›
Chain: C
Length: 249 amino acids
Theoretical weight: 28.83 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46675 (Residues: 318-560; Coverage: 27%)
Gene names: L2108, STU2, YLR045C
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 111.909Å b: 89.573Å c: 135.508Å
α: 90° β: 112.31° γ: 90°
R-values:
R R work R free
0.22 0.218 0.259
Expression systems:
  • Saccharomyces cerevisiae
  • Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Control of microtubule organization and dynamics: two ends in the limelight.
Akhmanova et al. (2015)
20 more

11 mentions without citation

Lysine acetylation of cytoskeletal proteins: Emergence of an actin code.
A et al. (2020)
10 more