› 4two

X-ray diffraction
2.05Å resolution

Human EphA3 Kinase domain in complex with compound 164

Released:
DOI: 10.2210/pdb4two/pdb
PDB entry 4two coloured by chain, front view.
PDB entry 4two coloured by chain, side view.
PDB entry 4two coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Analysis of the Binding of Type I, I1/2, and II Inhibitors to Eph Tyrosine Kinases.
Dong J, Zhao H, Zhou T, Spiliotopoulos D, Rajendran C, Li XD, Huang D, Caflisch A
ACS Med Chem Lett 6 79-83 (2015)
PMID: 25589935

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151400 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ephrin type-A receptor 3 Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 40.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29320 (Residues: 609-947; Coverage: 35%)
Gene names: EPHA3, ETK, ETK1, HEK, TYRO4
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 37W 1 x 37W
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 53.221Å b: 38.068Å c: 75.31Å
α: 90° β: 100.83° γ: 90°
R-values:
R R work R free
0.165 0.163 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Principles and Overview of Sampling Methods for Modeling Macromolecular Structure and Dynamics.
Maximova et al. (2016)
3 more

1 mention without citation

Structural Analysis of the Binding of Type I, I1/2, and II Inhibitors to Eph Tyrosine Kinases.
Dong et al. (2015)