› 4tqu

X-ray diffraction
3.2Å resolution

Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate

Released:
DOI: 10.2210/pdb4tqu/pdb
PDB entry 4tqu coloured by chain, front view.
PDB entry 4tqu coloured by chain, side view.
PDB entry 4tqu coloured by chain, top view.
Source organism: Sphingomonas sp.
Primary publication:
Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate.
Maruyama Y, Itoh T, Kaneko A, Nishitani Y, Mikami B, Hashimoto W, Murata K
Structure 23 1643-1654 (2015)
PMID: 26235029

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-192159 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
ABC transmembrane type-1 domain-containing protein Chain: M
Molecule details ›
Chain: M
Length: 301 amino acids
Theoretical weight: 34.29 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT8 (Residues: 25-324; Coverage: 93%)
Gene name: algM1
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
ABC transmembrane type-1 domain-containing protein Chain: N
Molecule details ›
Chain: N
Length: 305 amino acids
Theoretical weight: 34.5 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT7 (Residues: 1-293; Coverage: 100%)
Gene name: algM2
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
ABC transporter domain-containing protein Chains: S, T
Molecule details ›
Chains: S, T
Length: 363 amino acids
Theoretical weight: 39.57 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT9 (Residues: 1-363; Coverage: 100%)
Gene name: algS
Sequence domains:
Structure domains:
Extracellular solute-binding protein Chain: Q
Molecule details ›
Chain: Q
Length: 516 amino acids
Theoretical weight: 59.69 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT5 (Residues: 1-516; Coverage: 100%)
Gene name: algQ2
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: BEM, MAW
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 71.367Å b: 134.177Å c: 273.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.237 0.282
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mechanistic diversity in ATP-binding cassette (ABC) transporters.
Locher KP. (2016)
4 more

5 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
4 more