› 4tq9

X-ray diffraction
1.49Å resolution

Crystal Structure of a GDP-bound G12V Oncogenic Mutant of Human GTPase KRas

Released:
DOI: 10.2210/pdb4tq9/pdb
PDB entry 4tq9 coloured by chain, front view.
PDB entry 4tq9 coloured by chain, side view.
PDB entry 4tq9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations.
Hunter JC, Manandhar A, Carrasco MA, Gurbani D, Gondi S, Westover KD
Mol Cancer Res 13 1325-35 (2015)
PMID: 26037647

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01116 (Residues: 1-167; Coverage: 88%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 65.32Å b: 41.427Å c: 115.119Å
α: 90° β: 105.09° γ: 90°
R-values:
R R work R free
0.166 0.164 0.194
Expression system: Escherichia coli BL21

Quick links

Citations

113 review citations

RAS Proteins and Their Regulators in Human Disease.
Simanshu et al. (2017)
112 more

11 mentions without citation

Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange.
Guillard et al. (2017)
10 more